• Jan 21, 2026 A260 280 following the DNA extraction protocol. Scenario 2: You are preparing RNA for a qPCR experiment. You obtain an A260/A280 ratio of 2.3. This high ratio could be due to the presence of carbohydrates or other contaminants, or RNA degradation leading to By Ned Dickens V
• Aug 30, 2025 A260 A280 Dna pure DNA sample typically exhibits an A260/A280 ratio between 1.8 and 2.0. A ratio below this range suggests contamination with proteins or other substances that absorb strongly at 280 nm. Conversely, a ratio significantly above 2.0 may indicate the presence of RNA contamination, By Rosalie Emard
